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1.
Jashanpreet Kaur Shu Yao Duan Lea A. I. Vaas Anahit Penesyan Wieland Meyer Ian T. Paulsen Helena Nevalainen 《PloS one》2015,10(3)
Genotyping studies of Australian Scedosporium isolates have revealed the strong prevalence of a recently described species: Scedosporium aurantiacum. In addition to occurring in the environment, this fungus is also known to colonise the respiratory tracts of cystic fibrosis (CF) patients. A high throughput Phenotype Microarray (PM) analysis using 94 assorted substrates (sugars, amino acids, hexose-acids and carboxylic acids) was carried out for four isolates exhibiting different levels of virulence, determined using a Galleria mellonella infection model. A significant difference was observed in the substrate utilisation patterns of strains displaying differential virulence. For example, certain sugars such as sucrose (saccharose) were utilised only by low virulence strains whereas some sugar derivatives such as D-turanose promoted respiration only in the more virulent strains. Strains with a higher level of virulence also displayed flexibility and metabolic adaptability at two different temperature conditions tested (28 and 37°C). Phenotype microarray data were integrated with the whole-genome sequence data of S. aurantiacum to reconstruct a pathway map for the metabolism of selected substrates to further elucidate differences between the strains. 相似文献
2.
大豆下胚轴可溶性蛋白中钙激活的蛋白激酶 总被引:6,自引:0,他引:6
大豆(Glycine m ax L.) 下胚轴可溶性蛋白提取液进行自磷酸化,以SDS-PAGE电泳分析其标记产物时发现,当有较高浓度的Ca2+ 存在于反应液中时,有一条18 kD蛋白带被高强度标记,同时也可观察到另一条标记强度不高的67 kD蛋白带. 当反应时间延长到15 或30m in 时,它们的标记强度都逐渐减弱,最终从放射自显影底片上消失;在反应液中加入钙螯合剂EGTA 时,则只有67 kD 被高强度标记;在磷酸化反应过程中加入非标记ATP,蛋白中的32P逐渐被非标记磷取代,表明反应体系处于磷酸化-脱磷酸化的平衡过程中,并有结果显示这一过程是钙依赖性的. 组蛋白H1 可以使反应进程加快,表明提取液中的蛋白激酶可以利用它作为底物. 综合结果表明,18 kD和67 kD蛋白可能是具有自磷酸化能力且对Ca2+ 敏感的蛋白激酶,它们对Ca2+ 的不同反应,使得钙信号的传递更具可控性 相似文献
3.
Ying Jin Dan Long Juan Li Ruichao Yu Yueming Song Jie Fang Xi Yang Shu Zhou Shishu Huang Zhihe Zhao 《Journal of cellular physiology》2019,234(9):14838-14851
Bone and tooth, fundamental parts of the craniofacial skeleton, are anatomically and developmentally interconnected structures. Notably, pathological processes in these tissues underwent together and progressed in multilevels. Extracellular vesicles (EVs) are cell-released small organelles and transfer proteins and genetic information into cells and tissues. Although EVs have been identified in bone and tooth, particularly EVs have been identified in the bone formation and resorption, the concrete roles of EVs in bone and tooth development and diseases remain elusive. As such, we review the recent progress of EVs in bone and tooth to highlight the novel findings of EVs in cellular communication, tissue homeostasis, and interventions. This will enhance our comprehension on the skeletal biology and shed new light on the modulation of skeletal disorders and the potential of genetic treatment. 相似文献
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Shu Ge Fukun Shi Guiyao Zhou Songhao Liu Zhiyun Hou Lu Peng 《Plasmonics (Norwell, Mass.)》2016,11(5):1307-1312
A surface plasmon resonance sensor based on a U-shaped photonic crystal fiber with a rectangular lattice has been designed through finite element method. The U-shaped fiber exhibits not only stronger mechanical strength but also better sensor performance than our previous scheme. The upper detection limit extends to higher analyze refractive index, 1.384, for phase interrogation. We introduce a ratio to evaluate the impact of higher order plasmonic mode. For wavelength modulation scheme, the parameter to describe the performance of a sensor is chosen to be the figure of merit, which can be up to 533.8[RIU?1] around complete coupling condition. 相似文献
7.
GABRIEL D. G. DEBOUT RICARDO PEREYRA BRENT C. EMERSON DOUGLAS W. YU 《Molecular ecology resources》2006,6(1):182-184
Fifteen microsatellite loci were isolated from the Peruvian tropical plant‐ant Allomerus octoarticulatus cf. demerarae (Hymenoptera: Myrmicinae) and their polymorphism was characterized. High levels of within‐population variation were observed at most loci, with number of alleles ranging from one to 21, and heterozygosity from 0 to 1 per population sample. Cross‐species amplification of these loci was also tested in one other species of the ant genus Allomerus (Allomerus decemarticulatus), displaying similar life history. 相似文献
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